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Title   한국인 위암 세포주에서 세포주기 조절 유전자의 발현 ( Expression of Cell Cycle Control Genes in Korean Gastric Cancer Cell Lines )
Publicationinfo   2000 Jan; 032(02): 279-288.
Key_word   Stomach neoplasm, Cell cycle control genes
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Abstract   Purpose: In order to elucidate the roles of the cell cycle control genes in the carcinogenesis of Korean gastric cancer, the expressions of cyclin A, Dl, E, p16INK4a and Rb in Korean gastric cancer cells were investigated in this study. Materials and Methods: The expression patterns of major cell cycle control genes in Korean gastric cancer cells (SNU1, 5, 216, 484, 601, 620, 638, 668, 710) were analysed by using western blot and reverse transcription-polymerase chain reaction (RT-PCR). Results: In most of the gastric cancer cells investigated, the overexpressions of cyclin A and hyperphosphorylated Rb, and markedly decreased expression of p16INK4a were observed. Over- expression of cyclin E was also observed in SNU1, 484, 601 and 638 gastric cancer cells. Conclusion: Considering all these findings, the following model of the gastric catcinogenesis caused by the altered expressions of the cell cycle control genes can be postulated: over- expressions of cyclin A and E cause acceleration of Rb phosphorylation, inactivation of Rb functions and acceleration of Gl/S transition of the cells; decreased expression of pl6INK4a causes inadequate inhibition of cyclin-CDK and further acceleration of Rb phosphorylation; consequently, Rb inactivation, Gl shortening, inappropriate cell division and decreased function of the maintenance of genomic stability occurs. All these abnormalities should contribute to the carcinogenesis of Korean gastric cancer cells.
저 자   임춘철(Chun Cheol Lim),김인호(In Ho Kim),조재위(Jae We Cho),백원기(Won Ki Baek),서성일(Seong II Suh),서민호(Min Ho Suh),박종욱(Jong Wook Park),강중신(Joong Shin Kang),손수상(Soo Sang Sohn)