논문검색
Author Bong kyung Moon, Min Chan Kim, Joo sung Park
Place of duty From the Departments of 1Family Medicine and 2Surgery, Dong-A University College of Medicine, Busan, Korea.
Title Synthetic CDCA Derivatives-Induced Apoptosis of Stomach Cancer Cell Line SNU-1 Cells
Publicationinfo Cancer Research and Treatment 2004 Apr; 036(02): 132-139.
Key_word Chenodeoxycholic acid,Stomach neoplasm,Apoptosis,Mitochondria,Caspases
Full-Text
Abstract Purpose: This study was conducted to explore whether CDCA derivatives induce apoptosis in a stomach cancer cell line, and to dissect the detailed mechanism underlying apoptosis. Materials and Methods: The human stomach cancer cell line, SNU-1, cells were treated with the synthetic CDCA derivatives, HS-1199 and HS-1200. DNA and mitochondrial stains were used to detect apoptotic cells by fluorescence imaging or flow cytometry. The caspase-3 activity was measured by Western blotting. Results: Both the HS-1199 and HS-1200 induced decreased viabilities of the SNU-1 cells, in time-dependent manners. The CDCA derivatives demonstrated various apoptosis hallmarks, such as mitochondrial changes (reduction of MMP, cytochrome c release, and Smac/ DIABLO translocation), activation of caspase-3 (resulting in the degradation of PARP and DFF45), DNA fragmentation and nuclear condensation. Conclusion: The CDCA derivatives, HS-1199 and HS- 1200, both induced apoptosis of the SNU-1 gastric cancer cells in caspase- and mitochondria-dependent fashions. Many important issues relating to their therapeutic applications remain to be elucidated. (Cancer Research and Treatment 2004;36:132-139)