논문검색
Author Jin Hwa Choi, M.D.1, Hong-Gyun Wu, M.D., Ph.D.2,4,5, Kyeong Cheon Jung, M.D., Ph.D.3, Seung Hee Lee, M.S.4, Eun Kyung Kwon, M.S.4,
Place of duty 1Department of Radiation Oncology, Chung-Ang University Hospital, Departments of 2Radiation Oncology, 3Pathology, Seoul National University College of Medicine, 4Cancer Research Institute, Seoul National University College of Medicine, 5Institute of Radiation Medicine, Medical Research Center, Seoul National University, Seoul, Korea
Title Apoptosis and Expression of AQP5 and TGF-Ղ in the Irradiated Rat Submandibular Gland
Publicationinfo Cancer Res Treat 2009 Sep; 041(03): 145-154.
Key_word Submandibular gland, Radiation, Apoptosis, AQP5, TGF-Ղ
Full-Text
Abstract Purpose: To evaluate the effect of X-ray irradiation on apoptosis and change of expression of aquaporin 5 (AQP5) and transforming growth factor-Ղ(TGF-Ղ) in the rat submandibular gland (SMG). Materials and Methods: SMGs of 120 male Sprague-Dawley rats were irradiated with a single X-ray dose (3, 10, 20, or 30 Gy). At the early and late post-irradiation phase, apoptosis was measured by the terminal deoxynucleotidyl transferase biotin-dUTP nick end labeling (TUNEL) method, and expression of AQP5 and TGF-Ղwas determined by immunohistochemical staining. Results: At the late post-irradiation phase, increased apoptosis was evident and marked decreases of expression of AQP5 expression by acinar cells and TGF-Ղexpression by ductal cells were evident. Conclusion: Apoptosis after X-ray irradiation develops relatively late in rat SMG. Irradiation reduces AQP5 and TGF-Ղexpression in different SMG cell types.
출판정보 대한암학회지 2009 Sep; 041(03): 145-154.